Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies.
Identifieur interne : 000745 ( Main/Exploration ); précédent : 000744; suivant : 000746Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies.
Auteurs : Xinwei Chen [Royaume-Uni] ; Dominika Lewandowska [Royaume-Uni] ; Miles R. Armstrong [Royaume-Uni] ; Katie Baker [Royaume-Uni] ; Tze-Yin Lim [États-Unis] ; Micha Bayer [Royaume-Uni] ; Brian Harrower [Royaume-Uni] ; Karen Mclean [Royaume-Uni] ; Florian Jupe [États-Unis] ; Kamil Witek [Royaume-Uni] ; Alison K. Lees [Royaume-Uni] ; Jonathan D. Jones [Royaume-Uni] ; Glenn J. Bryan [Royaume-Uni] ; Ingo Hein [Royaume-Uni]Source :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [ 1432-2242 ] ; 2018.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), Analyse de séquence d'ADN (MeSH), Cartographie chromosomique (MeSH), Diploïdie (MeSH), Gènes de plante (MeSH), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Marqueurs génétiques (MeSH), Phytophthora infestans (MeSH), Polymorphisme de nucléotide simple (MeSH), Résistance à la maladie (génétique), Solanum (génétique), Solanum (microbiologie).
- MESH :
English descriptors
- KwdEn :
- Chromosome Mapping (MeSH), DNA, Plant (genetics), Diploidy (MeSH), Disease Resistance (genetics), Genes, Plant (MeSH), Genetic Markers (MeSH), Phytophthora infestans (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Polymorphism, Single Nucleotide (MeSH), Sequence Analysis, DNA (MeSH), Solanum (genetics), Solanum (microbiology).
- MESH :
- chemical , genetics : DNA, Plant.
- genetics : Disease Resistance, Plant Diseases, Solanum.
- microbiology : Plant Diseases, Solanum.
- Chromosome Mapping, Diploidy, Genes, Plant, Genetic Markers, Phytophthora infestans, Polymorphism, Single Nucleotide, Sequence Analysis, DNA.
Abstract
KEY MESSAGE
A broad-spectrum late blight disease-resistance gene from Solanum verrucosum has been mapped to potato chromosome 9. The gene is distinct from previously identified-resistance genes. We have identified and characterised a broad-spectrum resistance to Phytophthora infestans from the wild Mexican species Solanum verrucosum. Diagnostic resistance gene enrichment (dRenSeq) revealed that the resistance is not conferred by previously identified nucleotide-binding, leucine-rich repeat genes. Utilising the sequenced potato genome as a reference, two complementary enrichment strategies that target resistance genes (RenSeq) and single/low-copy number genes (Generic-mapping enrichment Sequencing; GenSeq), respectively, were deployed for the rapid, SNP-based mapping of the resistance through bulked-segregant analysis. Both approaches independently positioned the resistance, referred to as Rpi-ver1, to the distal end of potato chromosome 9. Stringent post-enrichment read filtering identified a total of 64 informative SNPs that corresponded to the expected ratio for significant polymorphisms in the parents as well as the bulks. Of these, 61 SNPs are located on potato chromosome 9 and reside within 27 individual genes, which in the sequenced potato clone DM locate to positions 45.9 to 60.9 Mb. RenSeq- and GenSeq-derived SNPs within the target region were converted into allele-specific PCR-based KASP markers and further defined the position of the resistance to a 4.3 Mb interval at the bottom end of chromosome 9 between positions 52.62-56.98 Mb.
DOI: 10.1007/s00122-018-3078-6
PubMed: 29560514
PubMed Central: PMC5945768
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Armstrong</name><affiliation wicri:level="1"><nlm:affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Baker, Katie" sort="Baker, Katie" uniqKey="Baker K" first="Katie" last="Baker">Katie Baker</name><affiliation wicri:level="1"><nlm:affiliation>Synpromics, Edinburgh, EH25 9RG, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Synpromics, Edinburgh, EH25 9RG</wicri:regionArea><wicri:noRegion>EH25 9RG</wicri:noRegion></affiliation></author><author><name sortKey="Lim, Tze Yin" sort="Lim, Tze Yin" uniqKey="Lim T" first="Tze-Yin" last="Lim">Tze-Yin Lim</name><affiliation wicri:level="4"><nlm:affiliation>Columbia University, New York, NY, 10027, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Columbia University, New York, NY, 10027</wicri:regionArea><orgName type="university">Université Columbia</orgName><placeName><settlement type="city">New York</settlement><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Bayer, Micha" sort="Bayer, Micha" uniqKey="Bayer M" first="Micha" last="Bayer">Micha Bayer</name><affiliation wicri:level="1"><nlm:affiliation>The James Hutton Institute, ICS, Dundee, DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The James Hutton Institute, ICS, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Harrower, Brian" sort="Harrower, Brian" uniqKey="Harrower B" first="Brian" last="Harrower">Brian Harrower</name><affiliation wicri:level="1"><nlm:affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Mclean, Karen" sort="Mclean, Karen" uniqKey="Mclean K" first="Karen" last="Mclean">Karen Mclean</name><affiliation wicri:level="1"><nlm:affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Jupe, Florian" sort="Jupe, Florian" uniqKey="Jupe F" first="Florian" last="Jupe">Florian Jupe</name><affiliation wicri:level="1"><nlm:affiliation>Monsanto, Saint Louis, 63167, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Monsanto, Saint Louis, 63167</wicri:regionArea><wicri:noRegion>63167</wicri:noRegion></affiliation></author><author><name sortKey="Witek, Kamil" sort="Witek, Kamil" uniqKey="Witek K" first="Kamil" last="Witek">Kamil Witek</name><affiliation wicri:level="1"><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7GJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7GJ</wicri:regionArea><wicri:noRegion>NR4 7GJ</wicri:noRegion></affiliation></author><author><name sortKey="Lees, Alison K" sort="Lees, Alison K" uniqKey="Lees A" first="Alison K" last="Lees">Alison K. Lees</name><affiliation wicri:level="1"><nlm:affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation></author><author><name sortKey="Jones, Jonathan D" sort="Jones, Jonathan D" uniqKey="Jones J" first="Jonathan D" last="Jones">Jonathan D. Jones</name><affiliation wicri:level="1"><nlm:affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7GJ, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7GJ</wicri:regionArea><wicri:noRegion>NR4 7GJ</wicri:noRegion></affiliation></author><author><name sortKey="Bryan, Glenn J" sort="Bryan, Glenn J" uniqKey="Bryan G" first="Glenn J" last="Bryan">Glenn J. Bryan</name><affiliation wicri:level="1"><nlm:affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Scotland's Rural College (SRUC), Peter Wilson Building, West Mains Road, Edinburgh, EH9 3JG, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Scotland's Rural College (SRUC), Peter Wilson Building, West Mains Road, Edinburgh, EH9 3JG</wicri:regionArea><wicri:noRegion>EH9 3JG</wicri:noRegion></affiliation></author><author><name sortKey="Hein, Ingo" sort="Hein, Ingo" uniqKey="Hein I" first="Ingo" last="Hein">Ingo Hein</name><affiliation wicri:level="1"><nlm:affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK. Ingo.Hein@hutton.ac.uk.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Division of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee, DD2 5DA, UK. Ingo.Hein@hutton.ac.uk.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Life Sciences, Division of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee, DD2 5DA</wicri:regionArea><wicri:noRegion>DD2 5DA</wicri:noRegion></affiliation></author></analytic><series><title level="j">TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</title><idno type="eISSN">1432-2242</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosome Mapping (MeSH)</term><term>DNA, Plant (genetics)</term><term>Diploidy (MeSH)</term><term>Disease Resistance (genetics)</term><term>Genes, Plant (MeSH)</term><term>Genetic Markers (MeSH)</term><term>Phytophthora infestans (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Polymorphism, Single Nucleotide (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Solanum (genetics)</term><term>Solanum (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Cartographie chromosomique (MeSH)</term><term>Diploïdie (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Marqueurs génétiques (MeSH)</term><term>Phytophthora infestans (MeSH)</term><term>Polymorphisme de nucléotide simple (MeSH)</term><term>Résistance à la maladie (génétique)</term><term>Solanum (génétique)</term><term>Solanum (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term><term>Plant Diseases</term><term>Solanum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term><term>Maladies des plantes</term><term>Résistance à la maladie</term><term>Solanum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Solanum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term><term>Diploidy</term><term>Genes, Plant</term><term>Genetic Markers</term><term>Phytophthora infestans</term><term>Polymorphism, Single Nucleotide</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cartographie chromosomique</term><term>Diploïdie</term><term>Gènes de plante</term><term>Marqueurs génétiques</term><term>Phytophthora infestans</term><term>Polymorphisme de nucléotide simple</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>KEY MESSAGE</b></p><p>A broad-spectrum late blight disease-resistance gene from Solanum verrucosum has been mapped to potato chromosome 9. The gene is distinct from previously identified-resistance genes. We have identified and characterised a broad-spectrum resistance to Phytophthora infestans from the wild Mexican species Solanum verrucosum. Diagnostic resistance gene enrichment (dRenSeq) revealed that the resistance is not conferred by previously identified nucleotide-binding, leucine-rich repeat genes. Utilising the sequenced potato genome as a reference, two complementary enrichment strategies that target resistance genes (RenSeq) and single/low-copy number genes (Generic-mapping enrichment Sequencing; GenSeq), respectively, were deployed for the rapid, SNP-based mapping of the resistance through bulked-segregant analysis. Both approaches independently positioned the resistance, referred to as Rpi-ver1, to the distal end of potato chromosome 9. Stringent post-enrichment read filtering identified a total of 64 informative SNPs that corresponded to the expected ratio for significant polymorphisms in the parents as well as the bulks. Of these, 61 SNPs are located on potato chromosome 9 and reside within 27 individual genes, which in the sequenced potato clone DM locate to positions 45.9 to 60.9 Mb. RenSeq- and GenSeq-derived SNPs within the target region were converted into allele-specific PCR-based KASP markers and further defined the position of the resistance to a 4.3 Mb interval at the bottom end of chromosome 9 between positions 52.62-56.98 Mb.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29560514</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>03</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2242</ISSN><JournalIssue CitedMedium="Internet"><Volume>131</Volume><Issue>6</Issue><PubDate><Year>2018</Year><Month>Jun</Month></PubDate></JournalIssue><Title>TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</Title><ISOAbbreviation>Theor Appl Genet</ISOAbbreviation></Journal><ArticleTitle>Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies.</ArticleTitle><Pagination><MedlinePgn>1287-1297</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00122-018-3078-6</ELocationID><Abstract><AbstractText Label="KEY MESSAGE" NlmCategory="UNASSIGNED">A broad-spectrum late blight disease-resistance gene from Solanum verrucosum has been mapped to potato chromosome 9. The gene is distinct from previously identified-resistance genes. We have identified and characterised a broad-spectrum resistance to Phytophthora infestans from the wild Mexican species Solanum verrucosum. Diagnostic resistance gene enrichment (dRenSeq) revealed that the resistance is not conferred by previously identified nucleotide-binding, leucine-rich repeat genes. Utilising the sequenced potato genome as a reference, two complementary enrichment strategies that target resistance genes (RenSeq) and single/low-copy number genes (Generic-mapping enrichment Sequencing; GenSeq), respectively, were deployed for the rapid, SNP-based mapping of the resistance through bulked-segregant analysis. Both approaches independently positioned the resistance, referred to as Rpi-ver1, to the distal end of potato chromosome 9. Stringent post-enrichment read filtering identified a total of 64 informative SNPs that corresponded to the expected ratio for significant polymorphisms in the parents as well as the bulks. Of these, 61 SNPs are located on potato chromosome 9 and reside within 27 individual genes, which in the sequenced potato clone DM locate to positions 45.9 to 60.9 Mb. RenSeq- and GenSeq-derived SNPs within the target region were converted into allele-specific PCR-based KASP markers and further defined the position of the resistance to a 4.3 Mb interval at the bottom end of chromosome 9 between positions 52.62-56.98 Mb.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xinwei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lewandowska</LastName><ForeName>Dominika</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Armstrong</LastName><ForeName>Miles R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baker</LastName><ForeName>Katie</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Synpromics, Edinburgh, EH25 9RG, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>Tze-Yin</ForeName><Initials>TY</Initials><AffiliationInfo><Affiliation>Columbia University, New York, NY, 10027, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bayer</LastName><ForeName>Micha</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, ICS, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Harrower</LastName><ForeName>Brian</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McLean</LastName><ForeName>Karen</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jupe</LastName><ForeName>Florian</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Monsanto, Saint Louis, 63167, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Witek</LastName><ForeName>Kamil</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7GJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lees</LastName><ForeName>Alison K</ForeName><Initials>AK</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jones</LastName><ForeName>Jonathan D</ForeName><Initials>JD</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7GJ, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bryan</LastName><ForeName>Glenn J</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Scotland's Rural College (SRUC), Peter Wilson Building, West Mains Road, Edinburgh, EH9 3JG, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hein</LastName><ForeName>Ingo</ForeName><Initials>I</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-0128-2084</Identifier><AffiliationInfo><Affiliation>The James Hutton Institute, CMS, Errol Road, Dundee, DD2 5DA, UK. Ingo.Hein@hutton.ac.uk.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Division of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee, DD2 5DA, UK. Ingo.Hein@hutton.ac.uk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>BB/C007522/1</GrantID><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant><Grant><GrantID>BB/L008025/1</GrantID><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant><Grant><GrantID>BB/K018299/1</GrantID><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant><Grant><GrantID>Workpackages 2.x</GrantID><Agency>Rural and Environment Science and Analytical Services Division</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>03</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Theor Appl Genet</MedlineTA><NlmUniqueID>0145600</NlmUniqueID><ISSNLinking>0040-5752</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004171" MajorTopicYN="N">Diploidy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020641" MajorTopicYN="N">Polymorphism, Single Nucleotide</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032322" 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Armstrong</name><name sortKey="Baker, Katie" sort="Baker, Katie" uniqKey="Baker K" first="Katie" last="Baker">Katie Baker</name><name sortKey="Bayer, Micha" sort="Bayer, Micha" uniqKey="Bayer M" first="Micha" last="Bayer">Micha Bayer</name><name sortKey="Bryan, Glenn J" sort="Bryan, Glenn J" uniqKey="Bryan G" first="Glenn J" last="Bryan">Glenn J. Bryan</name><name sortKey="Bryan, Glenn J" sort="Bryan, Glenn J" uniqKey="Bryan G" first="Glenn J" last="Bryan">Glenn J. Bryan</name><name sortKey="Harrower, Brian" sort="Harrower, Brian" uniqKey="Harrower B" first="Brian" last="Harrower">Brian Harrower</name><name sortKey="Hein, Ingo" sort="Hein, Ingo" uniqKey="Hein I" first="Ingo" last="Hein">Ingo Hein</name><name sortKey="Hein, Ingo" sort="Hein, Ingo" uniqKey="Hein I" first="Ingo" last="Hein">Ingo Hein</name><name sortKey="Jones, Jonathan D" sort="Jones, Jonathan D" uniqKey="Jones J" first="Jonathan D" last="Jones">Jonathan D. Jones</name><name sortKey="Lees, Alison K" sort="Lees, Alison K" uniqKey="Lees A" first="Alison K" last="Lees">Alison K. Lees</name><name sortKey="Lewandowska, Dominika" sort="Lewandowska, Dominika" uniqKey="Lewandowska D" first="Dominika" last="Lewandowska">Dominika Lewandowska</name><name sortKey="Mclean, Karen" sort="Mclean, Karen" uniqKey="Mclean K" first="Karen" last="Mclean">Karen Mclean</name><name sortKey="Witek, Kamil" sort="Witek, Kamil" uniqKey="Witek K" first="Kamil" last="Witek">Kamil Witek</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Lim, Tze Yin" sort="Lim, Tze Yin" uniqKey="Lim T" first="Tze-Yin" last="Lim">Tze-Yin Lim</name></region><name sortKey="Jupe, Florian" sort="Jupe, Florian" uniqKey="Jupe F" first="Florian" last="Jupe">Florian Jupe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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